| Conjugate | HRP |
|---|---|
| Description | Pierce DAB Substrate Kit |
| Reactivity | Horseradish Peroxidase |
| Sufficient For | 25 Uses at 10 mL Each |
| Detection Method | Colorimetric |
| Form | Liquid |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Product Line | Pierce |
| Product Type | Substrate |
| Quantity | 275 mL |
| Substrate | Horseradish Peroxidase |
| Unit Size | Each |
Answer:The kit provides chromogenic detection of horseradish peroxidase (HRP).
Answer:The DAB reaction produces an insoluble brown precipitate.
Answer:It contains 25 mL of 10X DAB solution and 250 mL of Stable Peroxide Buffer.
Pierce™ DAB Substrate Kit provides a chromogenic substrate system for visualization of horseradish peroxidase activity in western blotting, immunohistochemistry, and related solid-phase detection methods. DAB reacts with HRP in the presence of peroxide to form an insoluble brown precipitate at the location of the enzyme-conjugated target. This 275 mL kit contains 25 mL of 10X DAB solution and 250 mL of Stable Peroxide Buffer, providing approximately 25 uses at 10 mL of working solution per use. The brown reaction product is insoluble in alcohol and many organic solvents, which makes it compatible with traditional tissue counterstaining and mounting workflows. Nitrocellulose and PVDF membranes are also compatible. Store the liquid kit at 4 °C and prepare the working substrate shortly before use.
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