Description
Quantity: 100 mL
Unit Size: Each
The combination of NBT (nitro-blue tetrazolium chloride) and BCIP (5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt) yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, a popular enzyme conjugate for antibody probes. Pierce NBT and BCIP powders are available individually and in two convenient, ready-to-use solutions (with and without a levamisole suppressor of endogenous phosphatase activity). Highlights:
• AP substrate– for detection of alkaline phosphatase (alk-phos) activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples
• Chromogenic– no special equipment needed for visualization; produces purple-black precipitate that is easy to photograph
• Package options– choose individual NBT and BCIP powders, or preformulated, ready-to-use solutions with or without levamisole suppressor 1-Step™ NBT/BCIP
• Ideal for immunoblotting applications
• Minimal assay-to-assay variability 1-Step NBT/BCIP Plus Suppressor
• Ideal for staining tissues assayed with calf intestinal alkaline phosphatase without the interference caused by endogenous phosphatases
• Contains 1mM levamisole to inhibit endogenous phosphatase activity