Thermo Fisher, 4304134, TaqMan™ Reverse Transcription Reagents

Name: TaqMan™ Reverse Transcription Reagents
Brand: Thermo Fisher
SKU: 4304134

No. of Reactions: 2000 Reactions at 10 μL, Unit Size: Each, TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)_16, and reverse primers are included for cDNA synthesis flexibility. These reagents are designed for reverse transcription and PCR amplification of a specific target RNA from either total RNA or mRNA. The reagents in this kit facilitate two-step RT-PCR, which is performed in two separate reactions and

Catalog Number: 4304134

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Category

One-Step RT-PCR Kits

TagsPCR Equipment and Supplies PCR Supplies Reverse Transcription Products cDNA Synthesis Kits and Master Mixes

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

No. of Reactions: 2000 Reactions at 10 μL
Unit Size: Each
Advantages of TaqMan Reverse Transcription Reagents
• Contains MultiScribe Reverse Transcriptase, a recombinant Moloney murine leukemia virus (MMLV) reverse transcriptase
• Includes random hexamers and oligo (dT)_16for flexibility
• Individual components provide versatility in assay setup
• Compatible with two-step RT-PCR reactionsUsing TaqMan Reverse Transcription Reagents
• TaqMan Reverse Transcription Reagents provide all the necessary components for reverse transcription of RNA to cDNA
• For two-step RT-PCR, random hexamers, oligo (dT)_16, and sequence-specific reverse primers can all be used
• The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems

Specifications

General

Final Product Type	First-Strand cDNA
Format	Kit
No. of Reactions	2000 Reactions at 10 μL
Optimal Reaction Temperature	42°C
Quantity	2000 Reactions
Reaction Format	Separate components
Reagent Type	Reverse Transcription
Reverse Transcriptase	MultiScribe
Ribonuclease H Activity	Yes
Shipping Condition	Dry Ice
Size (Final Product)	Up to 5 kb
Starting Material	RNA
Technique	Reverse Transcription
Concentration	50 U/μL
For Use With (Application)	Real Time PCR (qPCR)
Unit Size	Each

Ordering

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Unless otherwise stated, the minimum order amount is USD 1,000.
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