Thermo Fisher, 4334973, SYBR™ Green PCR Master Mix, 1 x 50 mL

Name: SYBR™ Green PCR Master Mix, 1 x 50 mL
Brand: Thermo Fisher
SKU: 4334973

Quantity: 1 x 50 mL, Unit Size: Each, Applied Biosystems™ SYBR™ Green Universal Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.

Catalog Number: 4334973

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Category

PCR Master Mixes

Pack Size1 x 50 mL

TagsPCR Equipment and Supplies PCR Supplies Real Time PCR Products Real Time PCR Master Mixes

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1 x 50 mL
Unit Size: Each
• SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required
• AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
• dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
• Proprietary buffer enhancements ensure performance and reliability
• Premixed components stored at 2° to 8°C significantly reduce assay setup timeMaximum Flexibility and Convenience:
• Applied Biosystems™ SYBR™ Green Universal Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required
• SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR
• The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity
• Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations
• This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation
• SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.
• Store at -20°C

Specifications

General

For Use With (Equipment)	7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Format	Tube
No. of Reactions	2000 Reactions
Passive Reference Dye	ROX (Pre-mixed)
Polymerase	AmpliTaq Gold DNA Polymerase
Product Line	SYBR
Product Type	Real Time PCR SYBR Master Mix
Quantity	1 x 50 mL
Sample Type	DNA (Genomic), cDNA
Shipping Condition	Wet Ice
Sufficient For	2000 Reactions of 50 μL
Volume	1 x 50 mL
Concentration	2X
Detection Method	SYBR
For Use With (Application)	Gene Expression
GC-Rich PCR Performance	High
Label or Dye	SYBR Green
PCR Method	qPCR
Reaction Speed	Standard
Unit Size	Each

Ordering

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