Thermo Fisher, 4337454, BigDye™ Terminator v3.1 Cycle Sequencing Kit, 24 Reactions
Quantity: 24 Reactions, Unit Size: Each
Catalog Number: 4337454
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Category
Pack Size24 reactions
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| For Use With (Application) | AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), Mixed-Base Detection, Long-Read Sequencing, Resequencing, De Novo Sequencing - High Throughput (cDNA), GT-Rich or Difficult Template Sequencing |
|---|---|
| For Use With (Equipment) | SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers |
| Product Line | BigDye™ Terminator |
| Product Type | Sequencing Buffer |
| Quantity | 24 Reactions |
| Technique | Fluorescent Dye Terminator Sequencing |
| Template Compatibility | BAC DNA, Lambda DNA, Genomic DNA (Bacterial), Single Stranded DNA, Rolling Circle Amplified Products, Fosmids, PCR Amplicons (50:50 Heterozygotes), Plasmid DNA (≤15 Kb), PCR Amplicons |
| Format | 96- or 384-well Plate |
| Unit Size | Each |
Answer:This configuration supports 24 reactions.
Answer:The kit uses fluorescent dye-terminator Sanger sequencing chemistry.
Answer:It includes Ready Reaction Mix, M13 (-21) primer, pREF-BDT control DNA, and 5X Sequencing Buffer.
BigDye™ Terminator v3.1 Cycle Sequencing Kit provides fluorescent dye-terminator chemistry for Sanger sequencing of PCR products, plasmids, fosmids, BACs, and other compatible DNA templates. This configuration supports 24 reactions and includes 200 µL of BigDye Terminator v3.1 Ready Reaction Mix, M13 (-21) primer, pREF-BDT control DNA, and 1 mL of 5X Sequencing Buffer. The v3.1 chemistry is optimized for long read lengths, improved dye mobility, and reliable performance across a wide range of sequencing applications. It is used for de novo sequencing, resequencing, mutation analysis, and finishing workflows before capillary electrophoresis. The kit is stored at −15 to −25 °C, while the sequencing buffer may be refrigerated.
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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