Thermo Fisher, 4371353, TaqMan™ Genotyping Master Mix, 1 x 1 mL

Name: TaqMan™ Genotyping Master Mix, 1 x 1 mL
Brand: Thermo Fisher
SKU: 4371353

Quantity: 1 x 1 mL, Unit Size: Each

Catalog Number: 4371353

Pricing

Send an RFQ.

View RFQ List

Category

qPCR Master Mixes

Pack Size1 x 1 mL

TagsPCR Equipment and Supplies PCR Supplies Real Time PCR Products Real Time PCR Master Mixes

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1 x 1 mL
Unit Size: Each
• Distinct clusters and high call rates for unambiguous allelic discrimination
• Validated with TaqMan™ SNP Genotyping Assays
• Excellent pre- and post-PCR stability for high throughput setup and analysis
• Reaction size is based on a 50μL reaction volumeMay be used for a variety of genotyping applications, including:
• Candidate gene studies
• Drug-target validation
• Disease association studies
• Population genetics
• Linkage mapping
• Agricultural applicationsExceptional Performance from an Optimized Mix (2X)Accurate genotype assignments result from preferential binding of the allele-specific probe to the matching target. TaqMan™ Genotyping Master Mix enables specific binding of the probe to achieve exceptional cluster resolution. Key features include:
• AmpliTaq Gold™ DNA Polymerase, UP (Ultra Pure) - Automatic hot start enzyme designed to be active during thermal cycling and inactive at room temperature for easy reaction setup
• Optimized mix components provide excellent specificity for discrimination between alleles
• Passive internal reference based on proprietary ROX™ dye for increased precision on Applied Biosystems™ real-time PCR instruments
• Single thermal cycling condition for consistent results with TaqMan™ AssaysValidated with TaqMan™ Genotyping Assays and Instruments:
• TaqMan™ Genotyping Master Mix has been tested across all types of TaqMan™ SNP Genotyping Assays: TaqMan™ Drug Metabolism Genotyping Assays, TaqMan™ SNP Genotyping Assays, and Custom TaqMan™ SNP Genotyping Assays
• In addition, the master mix is validated with the Applied Biosystems™ thermal cyclers and real-time PCR systemsHigh-Throughput Setup and Analysis:
• The combination of several low-cost thermal cyclers for PCR and a single real-time PCR instrument for allelic discrimination make high throughput SNP genotyping manageable
• TaqMan™ Genotyping Master Mix further improves high-throughput setup and analysis with excellent room temperature stability both before and after PCR
• This provides the flexibility required to run long experiments, unattended overnight or over a weekendReliable Discrimination with Challenging Targets
• Amplicon design constraints make SNP detection challenging, particularly when targets contain an abundance of GC- or AT-rich regions
• The optimized components of TaqMan™ Genotyping Master Mix result in tight, well-separated clusters and more accurate allele-calls compared to other mixes, even for challenging targetsSetting the Industry Standard for Quality:
• Extensive analytical and functional tests are performed for each manufactured lot of TaqMan™ Genotyping Master Mix, including one TaqMan™ SNP Genotyping Assay and one TaqMan™ Drug Metabolism Genotyping Assay
• Results are compiled in an informative Certificate of Analysis, which is accessible through the Applied Biosystems™ Web site

Specifications

General

Fidelity (vs. Taq)	2 X
For Use With (Equipment)	Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7
Genotyping Target	Copy Number Variants, SNPs (Unknown or Numerous), Drug Metabolizing Enzymes, SNPs (Known)
Passive Reference Dye	ROX (Pre-mixed)
Polymerase	AmpliTaq Gold DNA Polymerase
Product Line	TaqMan
Product Type	Genotyping Master Mix
Purity or Quality Grade	UP (Ultra Pure)
Quantity	1 x 1 mL
Sample Type	DNA (Genomic)
Technique	SNP Genotyping (Real-Time PCR-Based), CNV (Copy Number Variation)
Volume	1 mL
Concentration	2X
Detection Method	Primer-probe
For Use With (Application)	Genotyping
GC-Rich PCR Performance	High
PCR Method	qPCR
Reaction Speed	Standard
Unit Size	Each

FAQs

Question: What is the reagent concentration and pack size?

Answer:One 1 mL tube of 2X genotyping master mix is supplied.

Question: How many 50 µL reactions can it support?

Answer:It supports approximately 40 reactions at a 50 µL total volume.

Question: Does it include an allele-specific SNP assay?

Answer:No. A suitable TaqMan probe-and-primer genotyping assay must be supplied separately.

Application Note

TaqMan™ Genotyping Master Mix, 1 x 1 mL is designed for real-time PCR-based SNP genotyping and endpoint fluorescent allelic discrimination. The ready-to-use 2X mixture combines AmpliTaq Gold DNA Polymerase UP, deoxynucleotides without dUTP, passive reference dye and optimized reaction components for use with separately supplied TaqMan SNP Genotyping Assays. Genomic DNA and the appropriate allele-specific probe-and-primer assay are added to set up reactions in a compatible PCR instrument. Preferential binding of allele-specific probes creates distinct endpoint fluorescence clusters that allow discrimination among different genotypes. The 1 mL vial supports approximately 40 reactions at a total reaction volume of 50 µL because each reaction requires 25 µL of the 2X master mix; smaller reaction volumes can increase the number of tests. The formulation supports genetic variation studies including candidate-gene analysis, association studies and copy-number-related workflows using appropriate assays. It has pre- and post-PCR stability suited to plate preparation and endpoint measurement. Store refrigerated at 2–8°C; this is a master mix, not a complete SNP probe assay kit.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.