Description
Quantity: 72 tubes
Unit Size: Each
These microcentrifuge spin cups and spin columns are convenient tools for manipulating small volumes of affinity supports (5 to 500μL) for immunoprecipitation (IP) and pull-down purification assays. Five styles of polypropylene columns and matching collection tubes offer a variety of options to suit particular types of experimental needs. Add the affinity resin and sample to one of the columns and use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Spin columns allow you to affinity-purify more protein in less time. Highlights:
• Efficient washing of samples means fewer washes are needed to remove contaminating proteins
• Efficient elution of samples means better recovery of immunoprecipitation and assay products
• Filter cups and columns eliminate contamination of eluted samples with affinity resin
• Filter cups and columns eliminate the need to decant supernatant from pelleted debris or resin
• Spin protocols drastically reduce the time required for small-scale affinity protocols
• Low protein-binding polypropylene column construction minimizes nonspecific binding Spin Cups-Paper Filter with Collection Tubes (Part No. 69700):
• Column volume: 600μL
• Resin volume: 20 to 300μL
• Filter type: paper, 10μm pore size
• Cap type: collection tube cap fits onto inserted spin cup Spin Cups-Cellulose Acetate Filter with Collection Tubes (Part No. 69702):
• Column volume: 800μL
• Resin volume: 20 to 400μL
• Filter type: cellulose acetate, 0.45μm pore size
• Cap type: collection tube cap fits onto inserted spin cup
• Spin ColumnsScrew Cap with Luer-LokSpin Columns-Screw Cap (Part No. 69705):
• Column Volume: 900μL
• Resin volume: 20 to 400μL
• Filter type: polyethylene filter, 10μm pore size; small and large frit options for different sample sizes
• Cap type: O-ring screw top caps; press-in bottom plugs
• Luer-Lok Adapters enable use with syringe-based kitsSpin Columns-Snap Cap with Collection Tubes (Part No. 69725):
• Column volume: 1000μL
• Resin volume: 20 to 500μL
• Filter type: polyethylene filter, 30μm (20 to 50μm) pore size
• Cap type: snap cap on column (no cap on collection tube); press-on bottom capsMicro-Spin Columns (Part No. 89879):
• Column volume: 400μL
• Resin volume: 5 to 100μL
• Filter type: polyethylene filter, 30μm (20 to 50μm) pore size
• Cap type: O-ring screw top caps; press-on bottom capsMicrocentrifuge Tubes (Part No. 69715):
• 2mL capacity
• Dolphin-style bottom with attached snap cap
• Included and compatible with Part No. 69700Microcentrifuge Tubes (Part No. 69720):
• 2mL capacity
• Short, conical bottom with attached snap cap
• Included and compatible with Part No. 69702
