| Column Format | Analytical Column |
|---|---|
| Column Type | Reversed Phase |
| Endcapped | Yes |
| Max. Pressure | 5800 psi (400 bar) |
| Packing Material | Spherical, Fully Porous, Ultrapure Silica |
| Stationary Phase | C18 |
| Surface Area | 100 m²/g |
| Temperature | 60°C |
| USP Type | L1 |
| Carbon Load | 9% |
| Diameter (Metric) | 4.6 mm |
| Length (Metric) | 250 mm |
| Particle Size | 5 μm |
| Phase | Reversed Phase |
| Pore Size | 300 Å |
| Product Line | BioBasic |
| Technique | Bio-LC |
| Type | Analytical Column |
| pH | 2 to 9 |
| Unit Size | Each |
Answer:It is intended for peptides, small-to-medium proteins and other biomolecules.
Answer:The packing uses 300 Å pores and 5 µm fully porous silica particles.
Answer:This configuration is 250 mm long with a 4.6 mm internal diameter.
BioBasic™ 18 HPLC Column, 250 x 4.6 mm, 5 µm is used for reversed-phase separation of peptides, small-to-medium proteins and other biomolecules. The C18 stationary phase is bonded to ultrapure, fully porous silica with a 300 Å pore size, allowing biomolecules greater access to the stationary-phase surface than conventional small-pore materials. The 250 mm × 4.6 mm analytical format provides a long separation bed for complex biomolecular mixtures, while the 5 µm particles balance efficiency and pressure for standard HPLC operation. The packing is endcapped, has a surface area of about 100 m²/g and a carbon load of 9%. It operates over pH 2–9, supports temperatures up to 60°C and has a maximum pressure of 5800 psi (400 bar). The wide-pore C18 phase is compatible with LC/MS workflows and is suitable for protein and peptide characterization where reproducibility, peak capacity and robust reversed-phase retention are required.
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