Thermo Fisher, 7303982500, CaptureSelect™ HRP Anti-CD81 Conjugate

Name: CaptureSelect™ HRP Anti-CD81 Conjugate
Brand: Thermo Fisher
SKU: 7303982500

Quantity: 0.5 mL, Unit Size: Each, CaptureSelect HRP Anti-CD81 Conjugate includes a 12–15-kDa recombinant single-domain antibody fragment (VHH affinity ligand) that specifically binds with high affinity to the tetraspanin marker CD81. The affinity ligand is chemically conjugated to horseradish peroxidase (HRP) via an appropriate spacer to maintain full binding capacity. This conjugate allows you to detect, quantitate, and characterize CD81-expressing extracellular vesicles (EVs) like exosomes.

Catalog Number: 7303982500

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Category

Biotin-Conjugated Secondary Antibodies

Pack Size0.5 mL

TagsProtein Purification Protein Analysis Reagents Protein Purification Reagents and Kits

CollectionsThermo Fisher Protein Analysis Reagents for Western Blot, ELISA & Proteomics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 0.5 mL
Unit Size: Each
CaptureSelect HRP Anti-CD81 Conjugate can be combined with CaptureSelect Biotin Anti-CD81 Conjugate (Cat. No. 7103982100) in enzyme-linked immunoassays to quantify the CD81-expressing EVs. Sandwich ELISA setups can be designed using the biotin anti-CD81 conjugate as a capture reagent immobilized on a streptavidin-coated surface. HRP anti-CD81 conjugate is then used for detection of bound CD81-positive EVs.For detection of a broad variety of EVs and exosomes that originate from different cell types and secretion pathways, such as those of endosomal (CD63-enriched, CD9+, or CD81+) or ectosomal (CD63 low, CD9+, and CD81+) origin, we also offer CaptureSelect Biotin and HRP anti-CD63 conjugates and CaptureSelect Biotin and HRP anti-CD9 conjugates.Recommended working dilution for sandwich ELISA: 1:5000 to 1:10,000 (the optimal working dilution should be determined empirically for each application).

Specifications

General

Concentration	0.5 mg/mL
Final Product Type	HRP Conjugate
Formulation	PBS, 50% (v/v) glycerol
Label or Dye	Horseradisch peroxidase (HRP)
Ligand Type	Recombinant single-domain antibody fragment (V<sub>H</sub>H ligand)
Quantity	0.5 mL
Reactivity	Anti-tetraspanin CD81
Shipping Condition	Dry Ice
Target	Tetraspanin CD81
Form	Liquid
Product Line	CaptureSelect
Unit Size	Each

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