| Assay | ELISA |
|---|---|
| For Use With (Equipment) | Microplate Reader |
| Format | Kit |
| Quantity | 1 Assay |
| Target | VIISelect Affinity Ligand |
| Product Line | CaptureSelect |
| Type | ELISA Kit |
| Unit Size | Each |
Answer:It quantifies VII Select affinity ligand that may leach from Cytiva VII Select media during Factor VII purification.
Answer:The method is a sandwich-type ELISA with HRP/TMB colorimetric detection.
Answer:This configuration contains reagents for one assay.
CaptureSelect™ VII Select Leakage ELISA is a sandwich-format assay for quantifying ligand that may leach from Cytiva™ VII Select affinity media during Factor VII purification. This configuration contains reagents for one assay. The assay uses an anti-VII Select ligand coating reagent, a VII Select ligand standard, and a biotinylated anti-ligand detection reagent. Bound ligand is detected through a streptavidin-HRP and TMB colorimetric workflow, with absorbance measured at 450 nm after the reaction is stopped. Typical sensitivity is at or above 1 ng/mL, allowing the method to support process-development and downstream-purification monitoring where residual affinity ligand must be quantified. The core kit reagents are stored frozen at −20 °C, while several plate, blocking, HRP, and substrate materials are supplied separately.
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