Thermo Fisher, 88310, Pierce™ Kinase Enrichment Kit with ATP Probe

Quantity: 16 reactions
Unit Size: Each
These products, based on ActivX ATP and ADP Probes, feature an amine-reactive nucleotide analog and a desthiobiotin (biotin analog) tag that facilitates selective labeling of lysines in the kinase active site and then subsequent enrichment and recovery of labeled protein. These features allow identification and profiling of target enzyme classes across samples or assessment of the specificity and affinity of enzyme inhibitors.Highlights:
• Specific– label only the conserved active-site lysines of nucleotide-binding proteins
• Flexible– use for in vitro labeling of ATPase enzymes derived from cells or tissues
• Compatible– use with Western blot or mass spectrometry (MS) workflows Recommended for:Profile small-molecule binding affinities and active-site inhibition in a dose-dependent manner; Identify dozens to hundreds of inhibitor targets and off-targets from tissues, cells and subcellular proteomes; Enrichment of enzymes based on function.