Thermo Fisher, A12218, Amplex™ Red Phosphatidylcholine-Specific Phospholipase C Assay Kit

Name: Amplex™ Red Phosphatidylcholine-Specific Phospholipase C Assay Kit
Brand: Thermo Fisher
SKU: A12218

Quantity: 500 Assays, Unit Size: Each

Catalog Number: A12218

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Category

Cell-Free Protein Expression Kits

Pack Size500 assays

TagsCell Analysis Products Cell Based Assays Cell Function Reagents and Kits

CollectionsThermo Fisher Cell Analysis Products: Viability, Apoptosis, ROS & Calcium Imaging Assays Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 500 Assays
Unit Size: Each
The Amplex Red Phosphatidylcholine-Specific Phospholipase C Assay Kit provides a sensitive method for detecting phosphatidylcholine-specific phospholipase C (PC-PLC) activity using a fluorescence microplate reader or fluorometer. In this enzyme-coupled assay, PC-PLC activity is monitored indirectly using 10-acetyl-3,7-dihydroxyphenoxazine (Amplex Red reagent), a sensitive fluorogenic probe for hydrogen peroxide. PC-PLC converts phosphatidylcholine (lecithin) to phosphocholine and diacylglycerol. Alkaline phosphatase is added, which hydrolyzes phosphorylcholine to form choline. Choline is then oxidized by choline oxidase to form betaine and hydrogen peroxide. In the presence of horseradish peroxidase, the hydrogen peroxide reacts with Amplex Red reagent in a 1:1 stoichiometric ratio to generate the highly fluorescent product resorufin. Because resorufin has absorption and fluorescence emission maxima of approximately 571nm and 585nm, respectively, there is little interference from autofluorescence in most biological samples.
• Detects phospholipase-C activity levels as low as 0.2mU/mL
• Format allows for multiple time point measurements
• Designed for minimal autofluorescence interference
• Custom assay design and packaging are also availableUse Amplex Red Assays for a Broad Range of InvestigationsA wide variety of validated Amplex Red assays are available for studying cell signaling and lipids, neurobiology, inflammation and immune function, and metabolism. We also offer Amplex UltraRed Reagent, a second-generation reagent providing greater sensitivity and brighter fluorescence, and the Amplex Red/UltraRed Stop Reagent. The Amplex Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable for at least three hours.Enzyme and Protein Activity Assays, Lipase and Phopholipase Activity, Proteins, Expression, Isolation and Analysis

Specifications

General

Detection Method	Fluorescence
Dye Type	Other Label(s) or Dye(s)
Format	Cuvettes, 96-well plate
Quantity	500 Assays
Shipping Condition	Room Temperature
For Use With (Application)	Phospholipase Assay
For Use With (Equipment)	Fluorometer
Product Line	Amplex
Product Type	Amplex Red Assay Kit
Unit Size	Each

Ordering

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