Thermo Fisher, A14146, pJTI™ R4 Exp CMV EmGFP pA Vector

Name: pJTI™ R4 Exp CMV EmGFP pA Vector
Brand: Thermo Fisher
SKU: A14146

Quantity: 100 μg, Unit Size: Each

Catalog Number: A14146

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Category

ChIP Reagents & Kits

Pack Size100 µg

TagsMolecular Biology Reagents and Kits DNA Vectors

CollectionsThermo Fisher Molecular Biology Reagents & Kits Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 100 μg
Unit Size: Each
The pJTI™ R4 Exp CMV EmGFP pA vector is a positive control vector for assessing the success of a retargeting reaction with a Jump-In™ parental cell line. When co-transfected with the integrase vector (pJTI™ R4 Int vector) included in the Jump-In™ parental kits, the EmGFP will be expressed and positive cells will fluoresce green.Ensure the Success of Your Jump-In™ Retargeting ReactionsSuccessful retargeting of Jump-In™ parental cell lines like the the Jump-In™ GripTite™ HEK293 kit (A14150) is dependent on a variety of factors such as:Transfection efficiency
• Cell confluency
• Antibiotic selection conditions
• Quality and concentration of DNA
• Retargeting vector to integrase vector ratioWe strongly recommend including a retargeting reaction with the pJTI™ R4 Exp CMV EmGFP pA vector in your Jump-In™ experiment along with negative controls (no plasmid DNA, no integrase vector) so you can easily visualize the results and optimize the retargeting conditions.For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Specifications

General

Constitutive or Inducible System	Constitutive
Delivery Type	Transfection
For Use With (Application)	Stable Cell Line Development, Targeted Integration
Format	Liquid
Product Type	Mammalian Expression Vector
Quantity	100 μg
Selection Agent (Eukaryotic)	None
Shipping Condition	Dry Ice
Vector	Jump-In Vectors
Cloning Method	Gateway
Product Line	Jump-In
Promoter	CMV
Protein Tag	Untagged
Unit Size	Each

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