Thermo Fisher, A21174, GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit

Quantity: 10 reactions
Unit Size: Each
Simple, ready-to-use, all-in-one expression vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter. GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit is a vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter. The OFP reporter allows for fluorescence-based tracking of transfection efficiency, as well as FACS-based sorting/enrichment of Cas9 and CRISPR expressing cells. The linearized GeneArt™ CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease. A version with competent cells is also available (Cat. No. A21178). The GeneArt™ CRISPR Nuclease Vector system offers: • Easy-to-design genome engineering system • Affordable, ready-to-use cloning vectors • Enrichment for hard to transfect or difficult to modify cell linesFor research use only. Not for use in diagnostic procedures.