| Calibrated Dye | Cy™5, NED, TAMRA™ |
|---|---|
| Calibration Type | Spectral Calibration |
| For Use With (Application) | Spectral Calibration |
| For Use With (Equipment) | QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Pro, QuantStudio™ 7 Pro, QuantStudio™ 6 Flex (Software version 1.6 or later), QuantStudio™ 7 Flex (Software version 1.6 or later) |
| Format | 96-well, 0.2 mL |
| PCR Method | qPCR |
| Plate Compatibility | 96-well, 0.2 mL |
| Quantity | 1 Plate |
| Shipping Condition | Dry Ice |
| Product Type | Calibration Plate |
| Unit Size | Each |
Answer:The plate contains TAMRA, NED, and Cy5 dye formulations.
Answer:It is a 96-well calibration plate for 0.2 mL blocks.
Answer:It provides dye-specific spectral information used to correct fluorescence overlap in later qPCR runs.
QuantStudio™ 3/5 Spectral Calibration Plate 3 is used to establish the fluorescence signatures of TAMRA™, NED™, and Cy™5 dyes on compatible real-time PCR instruments. The plate is supplied preloaded in a 96-well, 0.2 mL format so the calibration can be run using the same optical geometry as routine qPCR plates. During calibration, the instrument measures each dye independently and generates spectral data used to correct signal overlap during subsequent singleplex and multiplex assays. This improves discrimination between reporter channels and supports more reliable quantitative fluorescence analysis. Plate 3 is used as part of the complete QuantStudio spectral calibration set for QuantStudio 3 and 5 systems and specified QuantStudio Pro or Flex workflows. The plate should remain frozen at approximately –20°C until use and should be handled following the instrument calibration workflow.
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