Thermo Fisher, A35495, VeriFiler™ Plus PCR Amplification Kit

Name: VeriFiler™ Plus PCR Amplification Kit
Brand: Thermo Fisher
SKU: A35495

Quantity: 200 Reactions, Unit Size: 200 reactions

Catalog Number: A35495

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Category

PCR Master Mixes

Pack Size200 reactions

TagsPCR Equipment and Supplies PCR Supplies Standard PCR Products Direct PCR Reagents and Kits

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 200 Reactions
Unit Size: 200 reactions
• A highly discriminating, highly sensitive, robust kit optimized for forensic casework
• Utilizing a 6-dye chemistry, the VeriFiler Plus kit targets 25 markers: 23 autosomal STRs, including Penta D, Penta E, and two gender discrimination markers
• These markers meet all of the requirements CODIS markers as well as the Chinese National autosomal DNA database
• Specifically developed to maximize sensitivity to enable maximum information recovery from challenging casework samples including touch, degraded, and inhibited samples
• It can also be run in direct mode for single source reference samples in database workflows
• The VeriFiler Plus kit includes all of the same markers as the VeriFiler Express and Huaxia Platinum kits, but in a complementary layout to maximize discrimination for degraded samples
• This kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support
• It is ISO18385 Forensic DNA Grade to provide piece of mind when using this kit for casework samples
• In addition to high performance casework, the VeriFiler Plus kit has also been validated with multiple sample collection devices such as treated paper, untreated paper, and swabs for direct amplification of single source samples
• This dual-use kit enables labs to more efficiently manage their inventory and analysis workflowsKey features of the VeriFiler Plus PCR Amplification Kit include:
• Very high discrimination powers across global populations
• Inclusion of all CODIS- and Chinese National database loci-required markers
• One kit for both casework and database applications
• Concordance with Huaxia Platinum and VeriFiler Express kits; the VeriFiler Plus kit contains the same loci as these databasing kits
• High sensitivity enabled by a high-volume sample load (up to 17.5 μL)
• 11 mini-STRs maximize information recovery from degraded DNA samples
• Internal Quality Control (IQC) system enables detection of and discrimination between amplification inhibition and DNA sample degradation
• Gender confirmation through two Y-chromosome markers
• Efficient workflow with 76-minute PCR cycle time

Specifications

General

Format	Tube
No. of Reactions	200 reactions
Product Line	VeriFiler
Product Type	PCR Amplification Kit
Quantity	200 Reactions
Shipping Condition	Dry Ice
Target Loci	AMEL, IQCL, IQCS, Penta D, Penta E, Yindel, D18S51, D5S818, D16S539, D3S1358, D2S1338, TPOX, FGA, D1S1656, D10S1248, D7S820, D13S317, CSF1PO, D22S1045, D6S1043, TH01, D2S441, D8S1179, vWA, D19S433, D12S391, D21S11
Label or Dye	VIC
Unit Size	200 reactions

FAQs

Question: How many reactions are included?

Answer:The kit contains sufficient reagents for 200 amplification reactions at 25 µL per reaction.

Question: How many autosomal STR loci are amplified?

Answer:The assay amplifies 23 autosomal STR loci together with additional sex-discrimination and quality-control targets.

Question: What do the two IQC markers provide?

Answer:They help distinguish PCR inhibition from DNA degradation and also serve as amplification quality controls.

Application Note

VeriFiler™ Plus PCR Amplification Kit is used for forensic human identification through multiplex amplification of short tandem repeat markers from casework and reference DNA samples. The six-dye assay amplifies 23 autosomal STR loci together with a Y insertion/deletion marker, Amelogenin, and two Internal Quality Control targets. The marker set includes the CODIS core loci as well as additional markers used in human identification databases. The two IQC targets help analysts distinguish amplification inhibition from DNA degradation, providing more information when interpreting difficult samples. The 200-reaction format is optimized for 25 µL amplification reactions and includes master mix, primer set, allelic ladder, and control DNA components. The chemistry is designed to recover information from challenging samples such as low-template, touch, degraded, or inhibited DNA while also supporting direct amplification workflows for suitable single-source reference samples.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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