Thermo Fisher, A36754, Neucode™ Lysine-202

Quantity: 25 mg
Unit Size: Each
• Thermo Scientific NeuCode amino acids augment the level of multiplexing achievable for the metabolic labeling of proteins for mass spectrometry analysis. NeuCode Lysine-202 (13C2 15N2 L-Lysine-2HCl) may also be used with traditional SILAC to improve flexibility of multiplexing options or to reduce complexity of analysis.
• Stable isotope labeling with amino acids in cell culture (SILAC) is a powerful method to identify and quantify relative differential changes in complex protein samples. NeuCode metabolic labeling is similar to SILAC, but differs in that the labeling only utilizes heavy amino acids. The increased multiplexing capability of NeuCode amino acids is possible through the use of mass defects from extra neutrons in the stable isotopes. These small mass differences may be resolved on high resolution mass spectrometers (Thermo Scientific™ Orbitrap™ Elite™, Orbitrap™ Fusion™ Tribrid™, and Orbitrap™ Fusion™ Lumos™ Tribrid™ mass spectrometers). Use of only heavy amino acids eliminates the need for 100% incorporation of amino acids used for SILAC (both heavy and light), and may be especially useful for studies with primary cells.
• NeuCode amino acids are used together with specialized cell culture media that are deficient in essential amino acids. Heavy L-lysine is used for SILAC analysis of peptides that have been digested with trypsin or LysC. NeuCode Lysine-202 may be used with 4,4,5,5-D4 L-Lysine for NeuCode duplex experiments and may be combined with light lysine and 13C6 15N2 L-Lysine-2HCl for SILAC three-plex experiments.Labeling efficiency—100% label incorporation into proteins of living cells without toxicityCompatible—may be multiplexed with existing SILAC amino acidsTime-saving—not necessary to label to 100% incorporation if only using heavy amino acidsHigh-quality supplements—heavy amino acids with >98% isotope purity