Description
Includes: Assay Kit with Sample Preparation
Unit Size: Each
This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ E. coli Residual DNA Quantitation system, enables highly sensitive quantitation of host cell DNA from E. coli expression systems, typically in less than four hours. The kit is part of an integrated system that includes sample preparation, TaqMan™ assay and master mix, standard DNA, instrument, and software. The system overcomes the limitations of traditional methods by combining high-recovery PrepSEQ™ sample preparation and TaqMan™ Assay-based quantitation of residual DNA.This kit is specifically designed for the Chinese market and manufactured at our Guangzhou site to enable local biopharmaceutical and biotech companies to leverage a highly sensitive, world-class residual DNA quantitation solution.This kit is available with or without sample preparation reagents in the form of a PrepSEQ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts.Features of the resDNASEQ Quantitative E. coli DNA Kit include:
• Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology
• Manual and automated sample preparation, optimized for quantitative recovery from complex sample matrices (Table 1)
• Consistent performance across the expected range of DNA fragment sizes
• Integrated system from sample to results with sample preparation, master mix, TaqMan™ primer/probe mix, and genomic DNA standardRapid, sensitive detection of E. coli DNAThe resDNASEQ E. coli Residual DNA Quantitation system is a qPCR-based system for the detection of host cell DNA from E. coli, an expression system commonly used for the production of recombinant proteins. Reliable and rapid, the system enables sensitive (LOQ = 15 pg DNA/mL test sample) and specific quantitation of E. coli DNA, typically in less than four hours. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to bulk drug substances—whether a sample contains high molecular weight or sheared DNA.
