Thermo Fisher, A45522, resDNASEQ™ Quantitative Vero DNA Kits

Name: resDNASEQ™ Quantitative Vero DNA Kits
Brand: Thermo Fisher
SKU: A45522

Includes: Assay Kit and Sample Preparation, Unit Size: Each, The resDNASEQ Quantitative Vero DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Vero (African Green Monkey) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of Vero cell DNA, typically in less than four hours. This performance helps ensure a high de

Catalog Number: A45522

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Category

PCR Cleanup Kits

TagsPCR Equipment and Supplies PCR Supplies Real Time PCR Products Real Time PCR Reagents and Kits

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Includes: Assay Kit and Sample Preparation
Unit Size: Each
Features of the resDNASEQ Quantitative Vero DNA Kit include:• Highly sensitive quantitation using proven TaqMan real-time qPCR technology• Offers consistent performance across the expected range of DNA fragment sizes• Integrated system includes sample preparation kit, master mix, TaqMan primer/probe mix, and Vero genomic DNA standard• Manual and automated sample preparation, optimized for quantitative recovery from complex sample matricesThis kit is available with or without sample preparation reagents in the form of a PrepSEQ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts.The resDNASEQ Quantitative Vero DNA Kit is designed to provide highly sensitive detection of Vero DNA, allowing you to use small sample volumes to help generate accurate results. The broad linear range of TaqMan real-time qPCR technology allows you to test samples with variable levels of Vero DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts.Easy to use, results in under 4 hoursThe easy workflow of the resDNASEQ Quantitative Vero DNA Kit consists of sample preparation, assay setup, and instrument run and data analysis—all of which you can perform typically in under 4 hours. Start by extracting DNA from your in-process purification and drug substance samples. Then perform qPCR analysis to compare the amount of Vero DNA in your test samples to a standard curve generated with known amounts of purified Vero genomic DNA standard. The resDNASEQ system includes almost everything you need to carry out these steps, from sample preparation kit to master mix to TaqMan primer/probe mix to Vero genomic DNA standard, as well as step-by-step instructions.Consistent performance even with fragmented DNAFor accurate quantitation of residual Vero DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. With the resDNASEQ system you get consistent assay performance across a range of DNA fragment sizes.

Specifications

General

Format	Kit
Host Cell	Vero
Includes	Assay Kit and Sample Preparation
No. of Reactions	100 Reactions
Product Line	resDNASEQ
Quantity	100 reactions
Detection Method	Primer-probe
Label or Dye	FAM
PCR Method	qPCR
Unit Size	Each

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