Thermo Fisher, A4983901, Viral-Plex™ Complexation Buffer

Name: Viral-Plex™ Complexation Buffer
Brand: Thermo Fisher
SKU: A4983901

Quantity: 100 mL, Unit Size: Each, Gibco Viral-Plex Complexation Buffer is a ready-to-use AAV production buffer and complexation reagent for gene therapy, formulated to efficiently complex plasmid DNA with lipid-based transfection reagents for viral vector manufacturing. This chemically defined, serum- and protein-free buffer provides an optimal environment for forming stable DNA/lipid complexes, enabling efficient transient transfection of Viral Production Cells 2.0 (VPC2.0). As a key component

Catalog Number: A4983901

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Category

Lipid-Based Transfection Reagents

Pack Size100 mL

TagsTransfection Reagents Biochemical Reagents Nucleic Acid Introduction Reagents

CollectionsThermo Fisher Biochemical Reagents for Buffers, Stains & Core Lab Work Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 100 mL
Unit Size: Each
Features of the Viral-Plex Complexation Buffer
• Chemically defined, animal origin–free formulation—protein- and phenol red–free buffer provides a controlled environment for consistent DNA–lipid complex formation
• Optimized for AAV workflows—designed for use with the AAV-MAX Production System to enable efficient, helper-free triple-transfection in suspension culture, streamlining gene therapy vector production
• High-titer, scalable production—supports AAV yields greater than 5 x 10^10vg/mL (unpurified) in suspension culture; seamlessly scales from shake flasks to bioreactors for large-scale viral vector manufacturing
• Ready-to-use solution—supplied as a 1X formulation (e.g., 100 mL bottle) requiring no preparation; mix with plasmid DNA for immediate transfection use
• Consistent performance—a stable, pre-formulated buffer ensures reproducible transfection efficiency and batch-to-batch consistency in viral vector manufacturingApplications
• Gene therapy—ideal for the production of adeno-associated viral (AAV) vectors for gene therapy research and development
• Cell therapy—supports the generation of adeno-associated viral (AAV) vectors for cell therapy applications, facilitating seamless transition from bench to clinic
• Research and development—provides a reliable and scalable solution for viral vector production in various research settingsAvailable in RUO or CTSOffered as both a Research Use Only (RUO) version and a GMP-compliant Cell Therapy Systems (CTS) version (Cat. No. A5145403) to support translational and clinical-scale applications.

Specifications

General

Cell Line	HEK293
Culture Type	Suspension Cell Culture
Description	Serum-free, animal origin-free, chemically defined, protein free, phenol red free
Expression System	Mammalian
For Use With (Application)	AAV Production
For Use With (Equipment)	AAV-MAX System
Includes	100 mL Gibco Viral-Plex Complexation Buffer
Product Line	AAV-MAX
Product Type	AAV production transfection buffer
Quantity	100 mL
Reagent Type	Transfection buffer
Shelf Life	12 months from date of manufacture
Shipping Condition	Wet Ice
Sterility	Sterile
Cell Type	Established Cell Lines
Format	Bottle
Sample Type	Plasmid DNA
Transfection Technique	Lipid-based Transfection
Unit Size	Each

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