Thermo Fisher, A56996, CTS™ Detachable Dynabeads™ CD3/CD28

Quantity: 10 mL
Unit Size: Each
Gibco CTS Detachable Dynabeads CD3/CD28 magnetic beads are intended for ex vivo isolation and activation of T cells in a simultaneous one-step mechanism. When used in combination with the CTS Detachable Dynabeads Release Buffer (Cat. No. A55883), this offering is the first-of-its-kind cGMP cell selection technology to have an active release mechanism for process development, clinical trial, and commercial manufacturing use.Features of the CTS Detachable Dynabeads CD3/CD28 include:
• Empower users with process flexibility by allowing active release of beads from target cells at any time
• Optimize target cell purity, yield, activation profile, and desired phenotype thanks to the active release mechanism that gives users more control
• Provide an automation-friendly solution by working directly with the CTS DynaCellect Magnetic Separation System
• Enable consistent performance in light of biological variability within processes
• Are suitable for both autologous and allogeneic cell therapy manufacturing processesCoated with camelid-derived single-domain VHH antibodies that target CD3 and CD28 cell surface markers, these Dynabeads magnetic beads provide both the primary and co-stimulatory signals that are required for activation and expansion of T cells. These Detachable Dynabeads are meant for direct use with the CTS Detachable Dynabeads Release Buffer that has an active ingredient, biotin, that competitively displaces a biotin derivative on the surface of the beads. This proprietary mechanism allows users to achieve active release of the beads from target cells at any time within their process.Based on a recommended 3-to-1 bead-to-target-cell ratio (not total nucleated cell number), one 10-mL vial can theoretically isolate and activate up to 1.33 x 10^9 T cells.CTS Detachable Dynabeads CD3/CD28 also include Drug Master Files (DMFs) to support cross-referencing in regulatory submissions prior to clinical trial and commercial manufacturing use.