Thermo Fisher, A66521, ATTO-TAG™ FQ Plus Amine-Derivatization Kit

Name: ATTO-TAG™ FQ Plus Amine-Derivatization Kit
Brand: Thermo Fisher
SKU: A66521

Quantity: 1 Kit, Unit Size: Each

Catalog Number: A66521

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Category

Protein Labeling Kits

TagsProtein Purification Protein Analysis Reagents Protein Purification Reagents and Kits

CollectionsThermo Fisher Protein Analysis Reagents for Western Blot, ELISA & Proteomics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1 Kit
Unit Size: Each
The ATTO-TAG FQ Plus Amine-Derivatization Kit is a comprehensive solution designed for the efficient and sensitive detection of primary amines. This kit is a modification of the ATTO-TAG FQ Amine Derivatization Kit (Cat. No. A2334), with a key improvement: potassium cyanide, a hazardous substance, has been replaced with mandelonitrile, a less-toxic alternative. This modification ensures a potassium cyanide-free workflow without compromising its performance.The ATTO-TAG FQ Plus Amine-Derivatization Kit reacts specifically with primary amines to form conjugates that can be analyzed by electrophoretic or chromatographic methods. This specificity ensures accurate and reliable results. The kit provides high sensitivity and excellent binding efficiency, making it suitable for a wide range of applications, including research, diagnostic, and forensic applications.The kit includes all necessary components for performing 200–400 derivatizations, depending on the conditions described in the protocols. The resulting products of FQ are maximally excited at 480 nm or by the 488 nm spectral line of the argon-ion laser and have emission maxima at 590 nm. This broad range of excitation and emission maxima enhances the kit's versatility and adaptability to various analytical methods.

Specifications

General

Chemical Reactivity	Amine
Excitation/Emission	480/590 nm
Format	Kit
Label or Dye	ATTO-TAG FQ
Quantity	1 Kit
Reactive Moiety	Aldehyde
Shipping Condition	Wet Ice
Target	Primary Amine
Product Line	ATTO-TAG
Type	Derivatization Kits and Reagents
Unit Size	Each

FAQs

Question: Which chemical groups are targeted by the derivatization?

Answer:The FQ chemistry reacts with primary amines to generate fluorescent conjugates for analytical separation and detection.

Question: What components and reaction capacity are supplied?

Answer:The kit includes 5 mg FQ reagent, 4 mL of 20 mM mandelonitrile in DMSO and 250 mg beta-cyclodextrin, sufficient for approximately 200–400 reactions.

Question: How should the kit be stored?

Answer:Store it frozen at −5 °C to −30 °C and protect the fluorescent reagent from light.

Application Note

The Invitrogen ATTO-TAG FQ Plus Amine-Derivatization Kit converts primary amines into fluorescent reaction products for sensitive chromatographic or electrophoretic detection. The kit contains 5 mg of ATTO-TAG FQ reagent, 4 mL of 20 mM mandelonitrile in DMSO, and 250 mg of beta-cyclodextrin. Mandelonitrile replaces the potassium cyanide reagent used in the earlier FQ formulation, but routine chemical precautions still apply. The supplied quantities support approximately 200–400 derivatizations, depending on the protocol and reaction scale. Typical fluorescent products can be excited near 480 nm or with a 488 nm laser and observed around 590 nm emission. Potential analytes include primary amine-containing peptides and other small molecules, subject to assay optimization. Dissolve and combine the components under the prescribed conditions, control incubation time and reaction pH, and protect light-sensitive material during preparation and measurement. Store the kit frozen between −5 °C and −30 °C, protected from light. The kit is intended for research and is not a standalone clinical diagnostic test.

Ordering

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