Description
Quantity: 5 nmol
Unit Size: Each
Invitrogen™ fluorescently labeled control siRNA for the easy monitoring of siRNA uptake by fluorescence microscopy along with siRNA-induced knockdown. Purified, annealed, and ready to use, the 5 nmol aliquot is supplied in a stable, dried format.
• Fluorescently labeled siRNAs simplify the monitoring of siRNA delivery
• Ideal for correlating siRNA uptake with mRNA knockdown
• Functionally tested in several common cell lines
• HPLC purified, duplexed, and ready to useVisualize siRNA Uptake with Labeled siRNAsFluorescent labeling of siRNAs allows direct observation of their cellular uptake, distribution, and localization. Fluorescently labeled siRNAs are commonly used to monitor transfection efficiency during optimization of transfection conditions. In general, siRNAs are rapidly taken up by and distributed throughout cells in as little as 4 hours.Correlate siRNA Uptake with Gene SilencingStudies show that the silencing activity ofSilencer™Cy™3 Labeled GAPDH siRNA is not affected by the fluorescent label. Therefore, these siRNAs are ideal for examining both siRNA uptake and siRNA-induced knockdown, and for correlating gene knockdown with transfection efficiency; this is important to know because different transfection reagents interact with siRNAs in different ways (i.e., some reagents may sequester the siRNA, resulting in poor gene knockdown). TheSilencer™Cy™3 Labeled GAPDH siRNA has the fluorescein derivative Cy™3 (excitation max. = 547, emission max. = 563 nm) on the 5' end of the sense strand, making it highly fluorescent when excited by the appropriate wavelength of light.Silencer™Cy™3 Labeled GAPDH siRNA is suitable for monitoring siRNA uptake by chemical transfection or electroporation. Cy™3 labeling is recommended over FAM™ labeling when using electroporation, because of its brighter fluorescence signal visible over a broader pH range. Electroporation produces a more diffuse siRNA distribution pattern, resulting in a less intense fluorescence signal than with chemical transfection.Accessory Products:Silencer™Cy™3 Labeled GAPDH siRNA (human) (SKU #AM4623) is also available asSilencer™FAM™ Labeled GAPDH siRNA for human (SKU #AM4622) and for human, mouse, and rat (SKU #AM4650).Silencer™ control siRNAs are unmodified 21-mers; they are designed to be used withSilencer™ siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Invitrogen™Silencer™ Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.
