Thermo Fisher, C400, Carboxy-H2DCFDA (general oxidative stress indicator)

Quantity: 25 mg
Unit Size: Each
6-carboxy-2',7'-dichlorodihydrofluorescein diacetate is chemically reduced, acetylated form of fluorescein used as indicator for reactive oxygen species (ROS) in cells. This nonfluorescent molecule is readily converted to green-fluorescent form when acetate groups are removed by intracellular esterases and oxidation (by the activity of ROS) occurs within cell. Upon cleavage of the acetate and ester groups by intracellular esterases and oxidation, both analogs form carboxydichlorofluorescein, with additional negative charges that impede its leakage out of the cell.
• Ex/Em: approx. 492 - 495/517–527nm
• Product is air sensitive (store under dry argon or nitrogen)
• Product may be dissolved in DMSO, DMF, or ethanol for use
• Indicator is cell permeant
• Fluorescence can be monitored using a flow cytometer, fluorometer, microplate reader, or fluorescence microscope, using excitation sources and filters appropriate for fluorescein
• Carboxylated H2DCFDA analog (carboxy-H2DCFDA), which has two negative charges at physiological pH, and its di(acetoxymethyl ester) available separatelyRefer to the Molecular Probes™ Handbook for additional product information.Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Nitro-Oxidative Stress, Reactive Oxygen Species