Thermo Fisher, C66455, TOP10 Electrocomp™ Kit, 5 x 100 μL

Name: TOP10 Electrocomp™ Kit, 5 x 100 μL
Brand: Thermo Fisher
SKU: C66455

Quantity: 5 x 100 μL, Unit Size: Each

Catalog Number: C66455

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Category

Phosphorylation Reagents

Pack Size5 x 100 µL

TagsCompetent Cells Protein Analysis Reagents

CollectionsThermo Fisher Protein Analysis Reagents for Western Blot, ELISA & Proteomics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 5 x 100 μL
Unit Size: Each
TOP10 Electrocomp E. coli cells are provided at a transformation efficiency of 1 x 10^10 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. This strain is used in wide range of applications and allows stable replication of high-copy number plasmids and maintenance of large plasmids. The TOP10 genotype is similar to the DH10B strain and features:• hsdR for efficient transformation of unmethylated DNA from PCR amplifications• mcrA for efficient transformation of methylated DNA from genomic preparations• lacZΔM15 for blue/white color screening of recombinant clones• endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by Endonuclease I• recA1 for reduced occurrence of non-specific recombination in cloned DNANotesPlease see the product manual for reaction scale recommendations. A high-voltage electroporation apparatus is required.GenotypeF^- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupGFind the strain and format that fits your needs• TOP10 is available in chemically competent and electrocompetent cell formats.• Choose MultiShot competent cells for high-throughput cloning applications.

Specifications

General

Antibiotic Resistance Bacterial	Yes (Streptomycin)
Blue/White Screening	Yes (<i>lacΔM15</i>)
Cloning Methylated DNA	Yes (<i>mcrA</i>)
Cloning Unstable DNA	Not suitable for cloning unstable DNA
Contains F' Episome	No
High-throughput Compatibility	Low
Improves Plasmid Quality	Yes (<i>endA1</i>)
Plasmid	May be used for plasmids >20 kb
Preparing Unmethylated DNA	No
Product Line	Electrocomp
Product Type	Electrocompetent Cells
Quantity	5 x 100 μL
Reduces Recombination	Yes (<i>recA1</i>)
Shipping Condition	Dry Ice
T1 Phage - Resistant (tonA)	No
Transformation Efficiency Level	High Efficiency (>1 x 10<sup>10</sup> cfu/μg)
Format	Tube
Species	E. coli (K12)
Unit Size	Each

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