Description
Quantity: 500 g
Unit Size: Each
Differentiate Enterobacteriaceae on the basis of double sugar fermentation and hydrogen sulphide production with Klinger Iron Agar. Non lactose fermenters initially produce a yellow slant and butt as a result of glucose fermentation. Once the glucose is depleted, the reactions reverts to red slants (alkaline) due to oxidation of acids. Lactose fermenting organisms produce yellow slants and butts. There is no reversion to red in the slant because enough acid is produced to maintain an acid pH under aerobic conditions. Non-fermenters produce red slants and butts. H 2 S production results in a blackening of the medium, either throughout the butt or in a ring formation near the top of the butt. Gas production is demonstrated by the presence of bubbles or cracks in the medium. Typical reactions observed on Kligler Iron Agar after 18-24 hours at 35°C. Organism Slope Butt Gas H_2S Shigella sonnei Red Yellow - - Shigella dysenteriae Red Yellow - - Salmonella typhi Red Yellow - + Salmonella species Red Yellow + + Enterobacter species Red Yellow + - Klebsiella species Yellow Yellow + - Escherichia coli Yellow Yellow + - Proteus mirabilis Red Yellow - + Morganella species Red Yellow V - Citrobacter freundii Yellow Yellow + + Yersinia species Red Yellow V - Not all products are available for sale in all territories. Please inquire. Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
