Thermo Fisher, CM1212B, Oxoid™ <i>Brilliance</i>™ Listeria Agar (ISO)

Name: Oxoid™ Brilliance ™ Listeria Agar (ISO)
Brand: Thermo Fisher
SKU: CM1212B

Quantity: 500 g, Yield: 7 L, Unit Size: Each, Isolate, enumerate and presumptively identify Listeria species and L. monocytogenes from food and environmental samples with the Thermo Scientific™ Oxoid™ Brilliance™ Listeria Agar (ISO) (Dehydrated culture medium and supplements) following broth enrichment. The medium identifies Listeria species based on their utilization of X-glucoside giving rise to blue-green colonies. Listeria monocytogenes are further differentiated by their ability to highly p

Catalog Number: CM1212B

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Category

Selective & Differential Media

Pack Size500 g

TagsMicrobiological Media and Media Additives Dehydrated Microbiology Media

CollectionsThermo Fisher Microbiological Media & Media Additives Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 500 g
Yield: 7 L
Unit Size: Each
Isolate, enumerate and presumptively identify Listeria species and L. monocytogenes with Thermo Scientific™ Oxoid™ Brilliance™ Listeria Agar (ISO)
• Prepare OxoidBrillianceListeria Agar (ISO) by adding Thermo Scientific™BrillianceListeria Agar (ISO) Selective Supplement (SR0257) and Thermo Scientific™ Oxoid™BrillianceListeria Agar (ISO) Selective Supplement (SR0258) to OxoidBrillianceListeria (ISO) Agar.
• Use as part of the Thermo Scientific™ Listeria Precis™ Detection and Enumeration method, or as part of the ISO 11290-1:2017 and ISO 11290-2:2017 reference methods for the detection and enumeration ofListeriaspecies andL. monocytogenes.
• Demonstrated to produce clearly differentiated colonies ofListeriawithin 22 hours when following the protocols for the Listeria Precis Methods or ISO 11290-1 and ISO 11290-2 methods. Oxoid Brilliance Listeria Agar uses the chromogen X-glucoside for presumptive identification of Listeria spp. This chromogen is cleaved by ß-glucosidase which is common to all Listeria species. Other organisms that possess this enzyme, such as enterococci, are inhibited by the selective agents within the medium; lithium chloride, polymyxin B and nalidixic acid, whilst amphotericin inhibits the growth of any yeasts and molds present in the sample. Listeria monocytogenes and pathogenic Listeria ivanovii are then further differentiated by their ability to produce the phospholipase enzyme, lecithinase. This enzyme hydrolyses the lecithin in the medium, producing an opaque white halo around the colony. The formulation and performance of Oxoid Brilliance Listeria Agar (ISO) conforms with ISO 11290-1:2017 and ISO 11290-2:2017 reference methods for the detection and enumeration of Listeria species and L. monocytogenes. Oxoid Brilliance Listeria Agar (ISO) has been validated as part of the Thermo Scientific™ Listeria Precis™ Methods for a broad range of samples according to ISO 16140-2 following NF validation rules when compared to the ISO 11290-1:2017 and ISO 11290 -2:2017.

Specifications

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FAQs

Question: What amount is supplied?

Answer:500 g of dehydrated agar base.

Question: What is the approximate prepared-medium yield?

Answer:About 7 L.

Question: How are presumptive Listeria colonies visualized?

Answer:Blue-green colonies are produced; L. monocytogenes may also show an opaque white halo.

Application Note

Oxoid™ Brilliance™ Listeria Agar (ISO) is a chromogenic medium base for isolation, enumeration, and presumptive differentiation of Listeria organisms from enriched food and environmental samples. This 500 g dehydrated configuration yields approximately 7 L of prepared medium when used with the required supplements. Listeria colonies develop a blue-green appearance through utilization of a chromogenic substrate, while presumptive L. monocytogenes colonies can produce an opaque white halo associated with lecithinase activity. The medium is intended for culture-based Listeria methods and supports selection of suspect colonies for confirmatory testing. The agar is supplied as dehydrated powder, not as ready-poured plates. The applicable additives, preparation conditions, and confirmation stages depend on the selected validated method.

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Thermo Fisher, CM1212B, Oxoid™ <i>Brilliance</i>™ Listeria Agar (ISO)

Send an RFQ.

Question: What amount is supplied?

Question: What is the approximate prepared-medium yield?

Question: How are presumptive Listeria colonies visualized?

Notice

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