Thermo Fisher, E12020, EnzChek™ Phosphatase Assay Kit

Name: EnzChek™ Phosphatase Assay Kit
Brand: Thermo Fisher
SKU: E12020

Quantity: 1000 Assays, Unit Size: Each

Catalog Number: E12020

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Category

ELISA Substrates

Pack Size1000 assays

TagsCell Analysis Products Cell Based Assays Cell Function Reagents and Kits

CollectionsThermo Fisher Cell Analysis Products: Viability, Apoptosis, ROS & Calcium Imaging Assays Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1000 Assays
Unit Size: Each
Using the EnzChek Phosphatase Assay Kit continuous monitoring of phosphatase activity, even at neutral or acidic pH, can be achieved. The kit contains Molecular Probes (proprietary) 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) substrate. DiFMUP is about 100 times more sensitive than 4-methylumbelliferyl phosphate (MUP) for the detection of prostatic acid phosphatase at pH 5.5. The kit is perfect for the continuous assay of prostatic acid phosphatase, protein phosphatase 1 or almost any other phosphatase that can be assayed with nonprotein-based substrates such as MUP or 4-nitrophenyl phosphate (PNPP). Each kit contains a sufficient amount of substrate for ∽1000 assays, using a reaction volume of 100μL per assay. Fluorescence (excitation/emission maxima approx. 358/455nm) can be measured in a fluorescence microplate reader or a standard fluorometer.Cell Analysis, Cellular Imaging, Drug Discovery and Development, Enzyme and Protein Activity Assays, High-Content Screening (HCS), Phosphatase Activity, Phosphatase Activity Assays, Phosphatase Biology, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation

Specifications

General

Detection Method	Fluorescence
Dye Type	DiFMU
Quantity	1000 Assays
Shipping Condition	Room Temperature
Substrate	DiFMUP
Substrate Properties	Chemical Substrate
Substrate Type	Phosphatase Substrate
Target Enzyme	Phosphatases
Color	Blue
Emission	360/460
For Use With (Application)	Phosphatase Assay
For Use With (Equipment)	Microplate Reader
Product Line	EnzChek
Product Type	EnzChek Assay Kit
Unit Size	Each

Ordering

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