Thermo Fisher, EC66552BOX, Novex™ IEF Protein Gels, pH 3-10, 1.0 mm

Name: Novex™ IEF Protein Gels, pH 3-10, 1.0 mm
Brand: Thermo Fisher
SKU: EC66552BOX

Wells: 12-well, Unit Size: Each, Novex™ pH 3-10 IEF Gels are used for pI determination and are excellent for native applications using soluble proteins. Isoelectric focusing (IEF) is an electrophoresis technique that separates proteins based on their isoelectric point (pI). The pI is the pH at which a protein has no net charge and does not move in an electric field. Vertical IEF gels effectively create a pH gradient so proteins separate according to their unique pI.

Catalog Number: EC66552BOX

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Category

Protein Transfer Buffers

TagsGel Electrophoresis Equipment and Supplies Precast Electrophoresis Gels Isoelectric Focusing Gels and Strips

CollectionsThermo Fisher Gel Electrophoresis: Agarose Gels, SDS-PAGE & Blotting Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Wells: 12-well
Unit Size: Each
IEG gels can be used to readily detect minor changes in a protein due to deamination, phosphorylation, or glycosylation, and can resolve different proteins of similar size that cannot be resolved on standard SDS-PAGE gels. Pre-focusing is not required. Total run time is approximately 2.5 hours.FormulationNovex™ IEF gels are 5% polyacrylamide and consist of high-purity acrylamide, bisacrylamide, TEMED, APS, ultrapure water, and 2% ampholytes. They do not contain denaturing reagents.Recommended buffersIEF is a sensitive technique which is affected by many factors. Our optimized, premixed IEF buffers reduce variability and help you obtain consistent results.

Specifications

General

For Use With (Equipment)	Mini Gel Tank
Gel Thickness	1.0 mm
Mode of Separation	pI (Charge)
Quantity	5 Gels/Box
Sample Loading Volume	20 μL
Shelf Life	6 Months
Shipping Condition	Wet Ice
Storage Requirements	Store at 2°C to 8°C. Do not freeze.
Width (Metric)	8 cm
Gel Percentage	5%
Gel Size	Mini
Gel Type	IEF
Product Line	Novex
Separation Type	Isoelectric Focusing
Wells	12-well
pH Range	3.5 to 8.5
Unit Size	Each

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