Description
Quantity: 10,000 units
Unit Size: Each
• Source:Aspergillus oryzae
• Molecular Weight: 29 kDa monomer Quality Control:
• The absence of contaminating double-stranded DNA specific nuclease activity confirmed by appropriate quality test
• Functionally tested for the generation of unidirectional deletions in DNA fragments (in conjunction with Exonuclease III) Source:
• Aspergillus oryzae Molecular Weight:
• 29 kDa monomer Definition of Activity Unit:
• One unit of the enzyme produces 1μg of acid soluble deoxyribonucleotides in 1 min. at 37°C
• Enzyme activity is assayed in the following mixture: 30 mM sodium acetate (pH 4.5), 50 mM NaCl, 0.1 mM ZnCl_2, 5% (v/v) glycerol, 800μg/ml heat denatured calf thymus DNA Storage Buffer:
• The enzyme is supplied in:20mM Tris-HCl (pH 7.5), 50mM NaCl, 0.1mM ZnCl_2and 50% (v/v) glycerol 5X Reaction Buffer:
• 200mM sodium acetate (pH 4.5 at 25°C), 1.5M NaCl and 10 mM ZnSO_4 Inhibition and Inactivation:
• Inhibitors: metal chelators, PP_i, P_i, 5'-ribonucleotides and deoxyribonucleotides
• Inactivated by heating at 70°C for 10 min. in the presence of EDTA Recommended for:Removal of single-stranded overhangs of DNA fragments (2); S1 transcript mapping (3, 4); Cleavage of hairpin loops; Creation of unidirectional deletions in DNA fragments in conjunction with Exonuclease III (5) Note:S1 Nuclease can introduce breaks into double-stranded DNA, RNA and DNA/RNA hybrids at high enzyme and low salt concentrations (6).
