Thermo Fisher, EP0133, SP6 RNA Polymerase, HC (≥100 U/μL)

Name: SP6 RNA Polymerase, HC (≥100 U/μL)
Brand: Thermo Fisher
SKU: EP0133

Quantity: 5, 000 units, Unit Size: Each, Thermo Scientific™ Bacteriophage SP6 RNA polymerase is DNA-dependent RNA polymerase with strict specificity for their respective double-stranded promoters.

Catalog Number: EP0133

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Category

DNA Polymerases

Pack Size000 units / pack

TagsRNAi and RNA Reagents RNA Polymerases

CollectionsThermo Fisher RNAi & RNA Reagents for Reliable Gene Silencing Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 5,000 units
Unit Size: Each
It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter.
• Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides) Recommended for:Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing. Note:Consensus promoter sequences: AATTAACCCTCACTAAA G GGAGA - T3 TAATACGACTCACTATA G GGAGA - T7 ATTTAGGTGACACTATA G AAGNG - SP6 The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively (9).

Specifications

General

Quantity	5,000 units
Polymerase	SP6 RNA Polymerase
Unit Size	Each

Ordering

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