Thermo Fisher, EP0282, Taq DNA Polymerase, native (5 U/μL)

Format: Without BSA
Unit Size: Each
Available with or without the stabilizing agent BSA, Taq DNA Polymerase, native, with BSA, is often considered the best choice when amplifying DNA samples of lower purity, e.g., genomic DNA from mouse tail.Both native Taq DNA polymerases are supplied with two buffers: 10X Taq Buffer with KCl and 10X Taq Buffer with (NH_4)_2SO_4. The latter allows for PCR at a wide range of magnesium concentrations and decreases unspecific primingFeatures
• Thermostable with half-life more than 40 min at 95°C
• Generates PCR products with 3'-dA overhangs
• Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescent-labeled nucleotides)Applications
• Routine PCR amplification of DNA fragments up to 5 kb
• DNA labeling
• High throughput PCRNotes
• The error rate ofTaqDNA Polymerase in PCR is 2.2 x 10^-5errors per nt per cycle. Accordingly, the accuracy of PCR is 4.5 x 10^4. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
• The 10XTaqBuffer without Detergent is recommended for microarray experiments.