Thermo Fisher, EP0404, <i>Taq</i> DNA Polymerase, recombinant (1 U/μL)

Name: Taq DNA Polymerase, recombinant (1 U/μL)
Brand: Thermo Fisher
SKU: EP0404

Quantity: 500 Units, Unit Size: Each, Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5' to 3' synthesis of DNA, has no detectable 3' to 5' exonuclease (proofreading) activity, and possesses low 5' to 3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Rec

Catalog Number: EP0404

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Category

DNA Polymerases

Pack Size500 units

TagsPCR Equipment and Supplies PCR Supplies Standard PCR Products Standard PCR Reagents and Kits

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 500 Units
Unit Size: Each
Taq DNA Polymerase is supplied with two buffers: 10X Taq Buffer with KCl and 10X Taq Buffer with (NH_4)_2SO_4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming.Features
• High thermostability with half-life greater than 40 min at 95°C
• Generates PCR products with 3'-dA overhangs
• Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescent-labeled nucleotides)Applications
• Routine PCR amplification of DNA fragments up to 5 kb
• High throughput PCR
• DNA labelingNotes
• The error rate ofTaqDNA Polymerase in PCR is 2.2 x 10^-5errors per nt per cycle. Accordingly, the accuracy of PCR is 4.5 x 10^4. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
• 10XTaqBuffer without Detergent is recommended for microarray experiments.

Specifications

General

Fidelity (vs. Taq)	1X
Hot Start	No
Overhang	3'-A
Polymerase	<i>Taq</i> DNA Polymerase
Product Type	<i>Taq</i> DNA Polymerase (recombinant)
Quantity	500 Units
Reaction Format	Separate Components
Concentration	1 U/μL
For Use With (Application)	Standard PCR
GC-Rich PCR Performance	Low
Reaction Speed	Standard
Unit Size	Each

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