Thermo Fisher, F23981, Fluo-4FF, AM, cell permeant - Special Packaging

Quantity: 10 x 50 μg
Unit Size: Each
Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca^2+. Fluo-5F, fluo-5N, and fluo-4ff are analogs of fluo-4 with lower Ca^2+-binding affinity, making them suitable for detecting intracellular calcium levels in the 1μM to 1 mM range that would saturate the response of fluo-3 and fluo-4. Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells. These indicators are compatible with excitation at 488 nm by argon-ion laser sources, making them useful for confocal microscopy, flow cytometry, and microplate screening applications.Calcium Indicator (AM Ester) Specifications:
• Label (Ex/Em of Ca^2+–bound form): Fluo-4ff (494/516 nm)
• Fluorescence intensity increase upon binding Ca^2+: >100 fold
• K_dfor Ca^2+in buffer: ∼9.7μM
• Exhibit fluorescence increase upon binding Ca^2+with little shift in wavelengthUsing TPEN to Control Heavy Metal CationsIn addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn^2+, Zn^2+, Pb^2+) with substantially higher affinity than Ca^2+. Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator TPEN.More Choices for Fluorescent Calcium IndicatorsWe offer a large selection of Molecular Probes™ calcium indicators for use in various experimental scenarios. For more information, review Fluorescent Ca^2+Indicators Excited with Visible Light—Section 19.3 in the Molecular Probes™ Handbook.For UV-excitable Ca^2+indicators, protein-based Ca^2+indicators, conjugates of Ca^2+indicators, and for fluorescence-based indicators of other metal ions (i.e., Mg^2+, Zn^2+) review Indicators for Ca^2+, Mg^2+, Zn^2+and Other Metal Ions—Chapter 19 in the Molecular Probes™ Handbook.For Research Use Only. Not for human or animal therapeutic or diagnostic use.