Thermo Fisher, N8080127, Random Hexamers (50 μM)
Quantity: 5 nmol, 100 μL, Unit Size: Each
Catalog Number: N8080127
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| For Use With (Application) | DNA Synthesis |
|---|---|
| Form | Liquid |
| Product Type | Primer |
| Quantity | 5 nmol, 100 μL |
| Shipping Condition | Dry Ice |
| Concentration | 50 μM |
| Primer | Random Hexamer |
| Unit Size | Each |
Answer:They are randomized six-base DNA oligonucleotides that prime nucleic-acid synthesis at multiple complementary template positions.
Answer:Yes. They are routinely used as reverse-transcription primers in cDNA synthesis and two-step RT-qPCR workflows.
Answer:A 100 µL solution at 50 µM, containing 5 nmol of random hexamer primers.
Invitrogen Random Hexamers are synthetic six-nucleotide DNA primers with randomized sequences for initiation of complementary DNA synthesis. The collection of short oligonucleotides anneals at multiple complementary positions along RNA templates, making it useful when reverse transcription must cover many RNA species or when an oligo(dT)-only strategy would be unsuitable. The 50 µM solution contains 5 nmol of primers in 100 µL and is commonly used for first-strand cDNA synthesis, two-step RT-qPCR and RT-PCR workflows. Include an appropriate reverse transcriptase, dNTPs and reaction buffer, and use the primer concentration specified by the selected protocol. Random priming can produce short cDNA fragments and may create rRNA-derived cDNA, so primer choice should match the downstream experiment. Store at −15 to −25°C and minimize unnecessary freeze-thaw cycles.
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