Thermo Fisher, F525L, Phire Reaction Buffers

Chemical Name or Material: Phire Reaction Buffer, detergent-free
Unit Size: Each
Phire Reaction Green Buffer includes a blue die that migrates with 3–5 kb DNA fragments in 1% agarose gel and a yellow dye that migrates faster than 10 bp DNA fragments in 1% agarose gel. The dyes have excitation peaks at 424 nm and 615 nm.The concentration of Mg^2+ in Phire Reaction Buffer is optimized to work well for most amplicons. The buffer contains 1.5 mM MgCl_2 at final reaction concentration. Separate tubes of MgCl_2 and DMSO are provided for further optimization.Notes
• For applications that require PCR product analysis by absorbance or fluorescence excitation, the colorless Phire Reaction Buffer is recommended.