Thermo Fisher, F548L, Phusion Flash High-Fidelity PCR Master Mix

Name: Phusion Flash High-Fidelity PCR Master Mix
Brand: Thermo Fisher
SKU: F548L

No. of Reactions: 500 Reactions, Unit Size: Each, Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time. Ready-to-use 2X master mix preserves the fidelity and the yield in reactions when using very short PCR protocols. The unique master mix composition permits usage of short cycling protocols with both low and high complexity DNA templates (15 seconds per kilobase or less). The master mix utilizes Phusion Flash II DNA Polymerase, a modified proofreading DN

Catalog Number: F548L

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Category

PCR Master Mixes

Pack Size548 L

TagsPCR Equipment and Supplies PCR Supplies Standard PCR Products High Fidelity PCR Reagents and Kits

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

No. of Reactions: 500 Reactions
Unit Size: Each
Features
• Extreme speed with extension times of 15 s/kb or less
• Hot start modification allows 'zero-time reactivation'
• Accurate proofreading DNA polymerase with a fidelity of 25XTaq
• High yields in reduced cycling time
• Minimized pipetting stepsApplications
• Fast PCR
• High-fidelity PCR
• Difficult (GC-rich) templates
• Template generation for sequencing Multiplex PCR
• Long-range PCR
• Cloning & mutagenesis
• MicroarrayNotes
• Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such asTaqDNA polymerases).

Specifications

General

Color	Colorless
Fidelity (vs. Taq)	25X
Hot Start	Built-In Hot Start
No. of Reactions	500 Reactions
Overhang	Blunt
Polymerase	Phusion Flash II DNA Polymerase
Product Type	High-Fidelity PCR Master Mix
Quantity	500 reactions
Reaction Format	SuperMix or Master Mix
Shipping Condition	Dry Ice
Size (Final Product)	20 kb or less
Concentration	2X
For Use With (Application)	High-fidelity PCR
GC-Rich PCR Performance	High
Reaction Speed	Fast
Unit Size	Each

FAQs

Question: How many reactions are included?

Answer:This configuration supports 500 reactions.

Question: What concentration is supplied?

Answer:The product is supplied as a ready-to-use 2X PCR master mix.

Question: What polymerase is used?

Answer:The master mix uses Phusion Flash II high-fidelity DNA polymerase.

Application Note

Phusion™ Flash High-Fidelity PCR Master Mix is a ready-to-use 2X PCR master mix designed for very fast high-fidelity amplification. This configuration supports 500 reactions and is supplied as five 1 mL tubes. The formulation uses Phusion Flash II DNA Polymerase, a modified proofreading polymerase derived from Phusion Hot Start II chemistry. Extension times of approximately 15 seconds per kilobase or less can be used with suitable templates, while the polymerase provides about 25-fold higher fidelity than Taq. The hot-start design enables rapid activation and supports difficult or GC-rich templates, long-range PCR, cloning, mutagenesis, sequencing-template preparation, and multiplex PCR. The enzyme generates blunt-ended PCR products.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
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