Thermo Fisher, F564L, Phusion U Multiplex PCR Master Mixes

Name: Phusion U Multiplex PCR Master Mixes
Brand: Thermo Fisher
SKU: F564L

Color: Green, No. of Reactions: 500 Reactions, Unit Size: Each, Thermo Scientific Phusion U Green Multiplex PCR Master Mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube. Over 20 primer pairs may be combined into a single reaction for highly specific and efficient multiplexing over a broad range of primer and template concentrations.

Catalog Number: F564L

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Category

PCR Master Mixes

Pack Size564 L

TagsPCR Equipment and Supplies PCR Supplies Standard PCR Products High Fidelity PCR Reagents and Kits

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Color: Green
No. of Reactions: 500 Reactions
Unit Size: Each
The Phusion U Multiplex PCR Master Mix contains a proprietary buffer with balanced concentrations of all PCR components eliminating the need for tedious optimization. The Green format further simplifies the workflow - it includes a density reagent and two electrophoresis tracking dyes for direct loading of PCR products on gels.Highlights
• Specific and efficient multiplex PCR of over 20 targets up to 2.5 kb
• Excellent results with templates of suboptimal purity due to inhibitor tolerance
• Fast cycling and direct loading of PCR products on gelsApplications
• Genotyping
• Pathogen detection
• Food testing
• Analysis of genetically modified organisms
• Amplification of microsatellitesThe master mix is based on Phusion U Hot Start DNA Polymerase, a high performance enzyme developed using fusion technology. Similarly to other enzymes from Phusion family, Phusion U DNA Polymerase incorporates more nucleotides per binding event than any other DNA polymerase. This allows achieving high yields of PCR products with shorter extention times and consequently reduced total protocol times. Significantly enhanced processivity also enables successful multiplex PCR on difficult targets such as GC-rich templates or templates of suboptimal purity. Being highly tolerant of many PCR inhibitors, Phusion U Multiplex PCR Master Mixes can even be used with unpurified samples such as blood or serum.Specificity of multiplex PCR is increased by Affibody-mediated hot start mechanism. Reversibly bound Affibody ligand inhibits the polymerase at ambient temperatures preventing amplification of nonspecific products and formation of primer-dimers.Using Phusion DNA PolymerasesAnnealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).

Specifications

General

Color	Green
Fidelity (vs. Taq)	25X
Hot Start	Built-In Hot Start
No. of Reactions	500 Reactions
Overhang	Blunt
Polymerase	Phusion U Hot Start DNA Polymerase
Product Type	Multiplex PCR Master Mix
Quantity	500 x 50 μL Reactions
Reaction Format	SuperMix or Master Mix
Shipping Condition	Dry Ice
Size (Final Product)	2.5 kb or less
Concentration	2X
For Use With (Application)	Hot-start PCR
GC-Rich PCR Performance	High
Reaction Speed	Fast
Unit Size	Each

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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