Thermo Fisher, F701, Mutation Generation System Kit

Name: Mutation Generation System Kit
Brand: Thermo Fisher
SKU: F701

Quantity: 10 reactions, Unit Size: Each, transposon products are based on the transposition machinery of the bacteriophage Mu. During the lytic phase of the phage's life cycle the machinery replicates its genome by transposing repeatedly inside the host genome. The Mu transposition reaction has been modified into an in vitro reaction catalyzed by a single enzyme - MuA Transposase. In this system, one in vitro reaction is capable of generating more than a million transposon insertion clones.

Catalog Number: F701

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Category

Sequencing Reaction Kits

Pack Size10 reactions

TagsPCR Equipment and Supplies PCR Supplies Standard PCR Products Direct PCR Reagents and Kits

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 10 reactions
Unit Size: Each
HighlightsThe Mutation Generation System (MGS Kit) and Stop Generation System (STOP Kit) were developed for functional analysis of proteins. These new transposon tools enable the creation of saturated libraries of mutated proteins in a single reaction with less hands-on time than any other method. The location of the transposon insertion in each mutated clone can be mapped by either PCR or sequencing. With MGS and STOP kits, thousands of mutated clones are ready for expression studies in just 2 to 3 days.The MGS Kit contains the complete set of reagents for transposon-based linker scanning mutagenesis of any target protein. The MGS Entranceposons are designed for making subtle changes in the structure of a target protein by inserting 15 bp in-frame linkers throughout the corresponding target gene. This in-frame insertion allows for conservation of downstream sequences.The STOPKit Entranceposons contain translational stop codons in all three reading frames within the terminal portion of the transposon sequence. The proprietary modification of the Stop Generation System makes it possible to generate a saturated C-terminal deletion library from virtually any target protein with a maximum addition of three amino acids.Highlights
• Efficient - Create saturated insertion libraries for sequencing and protein analysis in a single reaction
• Fast - Decrease hands-on time compared to conventional methods
• Random - Eliminate target site preference or insertion hot-spot

Specifications

General

Format	Kit
Mutagenesis Capability	Mutagenesis at Multiple Sites
No. of Reactions	10 reactions
Product Type	Mutation Generation System Kit
Quantity	10 reactions
Starting Material	DNA
Sufficient For	10 Reactions
Form	Solution
Reaction Speed	Fast
Unit Size	Each

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