Thermo Fisher, F702, Template Generation System II Kit

Name: Template Generation System II Kit
Brand: Thermo Fisher
SKU: F702

Quantity: 20 reactions, Unit Size: Each, Transposon technology is the first technology that allows scientist to insert constructed linear DNA fragment into circular or linear DNA target randomly and very efficiently. The Thermo Scientific Template Generation System II Kit (TGS II Kit) is based on the transposition reaction of the bacteriophage Mu. This system has been simplified to work in vitro, and an artificial Mu transposon, designated as the Entranceposon, has been constructed. The reaction

Catalog Number: F702

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Category

PCR Plates

Pack Size20 reactions

TagsPCR Equipment and Supplies PCR Supplies Standard PCR Products Direct PCR Reagents and Kits

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 20 reactions
Unit Size: Each
Highlights
• Provides templates for DNA sequencing more simply and with less hands-on time than any other method
• Thousands of ready-to-sequence templates from a single transposition reaction: Enough to sequence even large DNA clones (e.g. BAC clones) without fragmentation or subcloning
• Simple mapping of the Entranceposon insertions by colony-PCR or restriction enzyme digestion enables directed sequencing. Fewer sequencing reactions are required to complete a sequence than with the shotgun approach
• Universal primers: The sequencing and mapping primers are included in the kit, no need for custom primers.
• Bidirectional sequencing: A single template clone can be used for sequencing the flanking DNA on both sides of the Entranceposon insertion
• Three different antibiotic resistance genes are available in each TGS II KitAll components needed as well as detailed instruction manual to perform the reactions are included in the kit. TGS II Kit is an updated version of the TGS I Kit.

Specifications

General

Format	Tube
No. of Reactions	20 Reactions
Product Type	Template Generation System II Kit
Quantity	20 reactions
Starting Material	DNA
Sufficient For	20 Reactions
For Use With (Application)	Pre-amplification
Form	Solution
Reaction Speed	Fast
Unit Size	Each

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