Thermo Fisher, J60015.AC, Laemmli SDS sample buffer, reducing (4X)

Quantity: 25 mL
Unit Size: Each
General Description• Laemmli SDS sample buffer is composed of several compounds, including 375 mM Tris-HCl (pH 6.8), 9% SDS, 50% glycerol, 6% β-mercaptoethanol, 0.03% bromophenol blue• The SDS compound present in the Laemmli SDS sample buffer binds noncovalently to proteins. SDS is negatively charged and can mask the intrinsic charge of the protein it bindsApplication• Laemmli SDS sample buffer is used to denature proteins and for protein sample preparation to be used in the SDS-PAGE system• This sample buffer is used as an electrophoretic dye for denaturation of proteins and monitoring the front of the running gel• It is also used for polyacrylamide protein gel analysis