Thermo Fisher, K0702, GeneJET PCR Purification Kit, 250 preps
Quantity: 250 Preps, Unit Size: Each
Catalog Number: K0702
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Category
Pack Size250 preps
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| Elution Volume Max | 50 μL |
|---|---|
| Final Product Type | DNA |
| Format | Spin Column |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| Isolation Technology | Silica Spin Column |
| Purification Time | 5 min. |
| Quantity | 250 Preps |
| Run Time | 5 min. |
| Sample Type | PCR Reactions, Enzymatic Reactions (DNase digestions, RNA labeling and clean-up preps, IVT reactions) |
| Shipping Condition | Room Temperature |
| Starting Material Volume | ≤400 μL |
| Yield | 25 μg (Binding capacity) |
| For Use With (Application) | Southern Blotting |
| Unit Size | Each |
Answer:The kit contains materials for 250 preparations.
Answer:It can purify DNA fragments from approximately 25 bp to 20 kb.
Answer:Each spin column binds up to 25 μg of DNA.
GeneJET PCR Purification Kit, 250 preps is designed for rapid isolation of clean DNA fragments from PCR and other enzymatic reaction mixtures. The kit uses silica-based spin columns to bind DNA while washing away free primers, nucleotides, salts and enzymes that could interfere with subsequent molecular biology reactions. It can purify fragments approximately 25 bp–20 kb in length and offers up to 25 μg binding capacity per column. Typical recovery for fragments in the 100 bp–10 kb range is 90–100%, with a workflow taking approximately five minutes. Each preparation can start from a reaction volume of up to 400 μL, and the kit supports 250 separate preparations. Purified DNA is suitable for restriction digestion, cloning, sequencing, labeling, ligation, in vitro transcription and related downstream applications. The spin-column format avoids phenol/chloroform extraction and does not require handling loose purification resin. This configuration is a PCR cleanup product, rather than a gel-extraction kit specifically developed to dissolve and process excised agarose fragments. The silica column provides a straightforward cleanup step when PCR product purity or salt removal is important before analysis.
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