| Color | Colorless |
|---|---|
| Fidelity (vs. Taq) | 1X |
| Hot Start | No |
| Overhang | 3'-A |
| Polymerase | DreamTaq DNA Polymerase |
| Quantity | 4 x 1.25 mL |
| Reaction Format | SuperMix or Master Mix |
| Shipping Condition | Dry Ice |
| Concentration | 5 U/μL |
| For Use With (Application) | Standard PCR |
| GC-Rich PCR Performance | Low |
| Reaction Speed | Standard |
| Unit Size | Each |
Answer:It includes four 1.25 mL vials of colorless 2X master mix and four 1.25 mL vials of nuclease-free water.
Answer:Add template DNA and forward/reverse primers to the master mix.
Answer:No. The tracking dyes are included in the separate green formulation.
DreamTaq PCR Master Mixes (2X) is designed for routine PCR amplification using a ready-to-use, colorless 2X reaction mixture. This package contains four 1.25 mL vials of DreamTaq master mix and four 1.25 mL vials of nuclease-free water. The master mix combines DreamTaq DNA polymerase, its optimized reaction buffer, magnesium chloride and the four standard dNTPs; template DNA and primers are added separately. A standard 50 μL reaction uses 25 μL of 2X mixture to achieve a 1X working concentration. The formulation supports routine amplification of DNA fragments up to approximately 6 kb from genomic templates and up to 20 kb from viral DNA. It is suitable for conventional PCR, genotyping and preparation of PCR products for downstream analysis or TA cloning. Because this is the colorless format, it does not contain the electrophoresis tracking dyes added to the separate green version. The mixture generates PCR products with typical 3′ A overhangs and is not a hot-start high-fidelity formulation. It is shipped frozen and stored at −20 °C.
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