Thermo Fisher, K2562, Maxima H Minus Double-Stranded cDNA Synthesis Kit, 50 Reactions

Maxima H Minus Double-Stranded cDNA Synthesis Kit provides a complete workflow for generating double-stranded cDNA from total RNA or mRNA. First-strand synthesis uses Maxima H Minus Reverse Transcriptase, which has reduced RNase H activity and is useful when longer cDNA products are required. First- and second-strand synthesis can be performed sequentially in the same tube without intermediate organic extraction or ethanol precipitation. The 50-reaction configuration supports cDNA preparation for cloning, library construction and other downstream molecular-biology workflows that require double-stranded cDNA rather than a single first-strand product. The kit combines reverse-transcription and second-strand synthesis reagents in one system, reducing the need to assemble individual components. RNA quality and input amount remain important determinants of cDNA yield and length, so sample preparation should be matched to the downstream application. The same-tube workflow is especially useful when cDNA will be cloned or converted into libraries and minimizing transfer-related sample loss is a priority.