Thermo Fisher, MEPI500CA, EpiLife™ Medium, with 60 μM calcium

EpiLife™ Medium is a sterile serum-free basal medium formulated with 60 µM calcium chloride for culture of normal human epidermal keratinocytes and normal human corneal epithelial cells. This configuration contains 500 mL. The basal formulation provides essential and nonessential amino acids, vitamins, trace minerals, organic compounds, and inorganic salts but does not contain antibiotics, antimycotics, hormones, growth factors, or proteins. It is buffered with HEPES and bicarbonate and is designed for culture in a 5% CO₂ atmosphere. For long-term proliferation, the medium is supplemented with the appropriate keratinocyte or corneal epithelial growth supplement before use. The defined low-calcium environment helps support epithelial-cell growth while allowing the user to control added growth factors separately. Store at 4 °C protected from light and do not freeze the medium.