Description
Quantity: 50 μL
Unit Size: Each
Qdot™ 625 Streptavidin Conjugate comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Qdot™ nanocrystal). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays, and are also used in purification workflows to achieve targeted fractionation. Qdot™ nanocrystal conjugates are provided as 1μM solutions.Important Features of Qdot™ Streptavidin Conjugates:
• Streptavidin Qdot™ 625 conjugate has emission maxima of ∼625 nm
• Approximately 5 to 10 streptavidins per Qdot™ nanocrystal
• Extremely photostable and bright fluorescence
• Efficiently excited with single-line excitation sources
• Narrow emission, large Stokes shift
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and moreProperties of Qdot™ NanocrystalsThe Qdot™ streptavidin conjugate is the size of a large macromolecule or protein (∼15–20 nm) and represents the brightest class of streptavidin detection reagents. Qdot™ streptavidin conjugates are made from a nanometer-scale crystal of a semiconductor material (CdSe), which is coated with an additional semiconductor shell (ZnS) to improve the optical properties of the material. The Qdot™ 705 and Qdot™ 800 streptavidin conjugates, which include CdSeTe, are made in a similar fashion. This core-shell material is further coated with a polymer shell that allows the materials to be conjugated to biological molecules and to retain their optical properties.Other Fluorescent Conjugates of Streptavidin are AvailableWe offer several other Qdot™ colors or try theQdot™ Streptavidin Sampler Kit, containing Qdot™ streptavidin conjugates in six colors 525, 565, 585, 605, 655, and 705. In addition to nanocrystal conjugates, we offer a wide range of streptavidins conjugated to Alexa Fluor™ dyes, Oregon Green™ dye, enzyme conjugates, and traditional fluorophores like Texas Red™ dye, fluorescein (FITC) and more.Find Biotinylated ConjugatesWe offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies.
• Use theprimary antibody search toolto find biotinylated primary antibodies
• Use thesecondary antibody selector toolto find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodiesBlocking Endogenous BiotinNaturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try ourEndogenous Biotin-Blocking Kitto minimize this interference.Related Links:Learn more about Avidin-Biotin DetectionLearn more about Qdot™ Nanocrystals
