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Staphylococci are widespread in nature, although they are mainly found on the skin, skin glands and mucous membranes of mammals and birds. The coagulase-positive species, i.e. Staphylococcus aureus, is well documented as a human opportunistic pathogen. The ability to clot plasma continues to be the most widely used and accepted criterion for the identification of pathogenic staphylococci associated with acute infections^2. One of the pathogenic strains is methicillin-resistant strain. Methicillin resistance is associated with production of a unique penicillin-binding protein (PBP), 2a, which is bound and inactivated only at high concentrations of beta-lactam antibiotics. The heterogeneous expression of methicillin resistance by staphylococci poses problems in the detection of resistant strains^3.Use Mannitol Salt Agar with Oxacillin for selective and differential isolation of methicillin-resistant staphylococci.
• Easy-to-differentiate: Differentiates between coagulase-positive and coagulase-negative staphylococci.
• Selective: Sodium chloride in the concentration of 7.5% inhibits many bacteria other than staphylococci.
• Ready-to-use:Convenience of prepared media.This medium contains casein and meat peptones that supply nitrogen, amino acids, and peptides necessary for bacterial growth. Beef extract is a source of essential nutrients, carbon and sulfur for microbial growth. Phenol red is the pH indicator which causes a color change in the medium from red-orange to yellow when acid is produced. Colonies of Staphylococci that ferment mannitol will be surrounded by a yellow zone, while those that do not ferment mannitol will have no color change. Oxacillin (4μg/ml) is added to inhibit the growth of non-MRSA staphylococci and other susceptible organisms.Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.