Thermo Fisher, R37111, CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent

Name: CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent
Brand: Thermo Fisher
SKU: R37111

Quantity: 1.75 mL kit, Unit Size: Each

Catalog Number: R37111

Pricing

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Category

Cell Sorters

Pack Size1.75 mL

TagsCell Analysis Products Cell Based Assays Apoptosis Reagents and Kits

CollectionsThermo Fisher Cell Analysis Products: Viability, Apoptosis, ROS & Calcium Imaging Assays Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1.75 mL kit
Unit Size: Each
Activation of caspase-3 is an early indicator of apoptosis.
• Allows rapid and sensitive detection of cells destined for cell death
• Formulated as room temperature-stable solution and comes in convenient dropper bottle: tip and drip2 drops/mL to stain cells
• Optimized caspase-3/7 substrate for apoptosis analysis
• Simple and fast no-wash protocol helps preserve delicate apoptotic cells
• Compatible with both live-cell fluorescence imaging and formaldehyde-based fixation methods
• Ready-to-use liquid formulation: no need to dilute, weigh, or pipet
• Four amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye that is nonfluorescent when not bound to DNA: The Caspase-3/7 Green reagent is intrinsically nonfluorescent, as DEVD peptide inhibits binding of dye to DNA
• Upon activation of caspase-3/7 in apoptotic cells, DEVD peptide is cleaved and free dye can bind DNA, generating bright green fluorescence
• Apoptotic cells with activated caspase-3/7 will have bright green nuclei, while cells without activated caspase 3/7 will show minimal fluorescence
• Fluorescent signal survives fixation and permeabilization, allowing end-point assays and immunocytochemistry-based methods
• Optimization may be needed for some cell types, conditions, and applications: In such cases, simply add more or fewer drops until optimal staining intensity is obtained
• If desired, combine with red or far-red nuclear stain for total cell count
• Excited with maximum at 502nm and has emission maximum at 530nm
• It is detected through standard GFP and FITC filtersApoptosis, Apoptosis Related Factors, Caspase Activation, Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, High-Content Screening (HCS)

Specifications

General

Color	Green
Compatible Cells	Mammalian Cells
Excitation/Emission	502/530
For Use With (Application)	Cell Imaging
For Use With (Equipment)	Confocal Microscope, Floid™ Cell Imaging System, Fluorescence Microscope, Flow Cytometer, Floid™ Cell Imaging System
Label Type	Other Label(s) or Dye(s)
Product Line	CellEvent, ReadyProbes
Product Type	Caspase 3/7 Reagent
Quantity	1.75 mL kit
Detection Method	Fluorescence
Format	Dropper Bottle
Unit Size	Each

Ordering

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Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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