Thermo Fisher, S1264, SBFI, AM, cell permeant - Special Packaging

Name: SBFI, AM, cell permeant - Special Packaging
Brand: Thermo Fisher
SKU: S1264

Quantity: 20 x 50 μg, Unit Size: Each

Catalog Number: S1264

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Category

Cell Imaging Plate Readers

TagsCell Analysis Products Cell Based Assays Cell Function Reagents and Kits

CollectionsThermo Fisher Cell Analysis Products: Viability, Apoptosis, ROS & Calcium Imaging Assays Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 20 x 50 μg
Unit Size: Each
• In combination with other fluorescent indicators used to correlate changes in intracellular Na^+with Ca^2+and Mg^2+concentrations, intracellular pH, and membrane potential
• Although selectivity of SBFI for Na^+is less than that of calcium indicators such as fura-2, it is sufficient for detection of physiological concentrations of Na^+in presence of other monovalent cations
• Spectral response of SBFI upon ion binding permit excitation ratio measurements, and this indicator can be used with same optical filters and equipment used for fura-
• Label (Ex/Em): SBFI (approximately 340,380/500nm)
• Lyophilized product may be dissolved in DMSO for use
• Product is typically loaded into cells by adding dissolved indicator to medium containing cells
• Selectivity Considerations and Cell Loading Strategies
• Dissociation constant (K_d) of SBFI for Na^+is 3.8mm in absence of K^+, and 11.3 mm in solutions with combined Na^+and K^+concentration of 135 mm (which approximates physiological ionic strength)
• SBFI is approximately 18-fold more selective for Na^+than for K^+Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Cell-Based Ion Channel Assays, Drug Discovery and Development, Ion Channel Biology, Ionic Homeostasis and Signaling, Sodium and Potassium Detection, Target and Lead Identification and Validation

Specifications

General

Detection Method	Fluorescence
Dye Type	Sodium Indicator
Quantity	20 x 50 μg
Shipping Condition	Room Temperature
For Use With (Application)	Cell Viability and Proliferation
For Use With (Equipment)	Fluorescence Microscope
Product Type	SBFI AM
Unit Size	Each

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