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Answer:Lecithinase and lipase-related reactions in suitable culture media, including visible precipitation around colonies.
Answer:One 100 mL sterile emulsion; store at 2–8°C.
Answer:No. Egg Yolk Emulsion is a separate supplement; tellurite-containing formulations are different products.
Thermo Scientific Oxoid Egg Yolk Emulsion is a ready-to-use sterile enrichment supplement for microbiological culture media. The 100 mL preparation supplies egg-yolk components that support examination of lecithinase and lipase activity in organisms such as Bacillus, Clostridium and Staphylococcus species. On appropriate agar formulations, enzymatic reactions can produce zones of opacity or other characteristic changes around growing colonies. Add the specified volume to the chosen sterile agar base after cooling it to the temperature required for supplementation; avoid overheating the emulsion or introducing contamination. Prepare the final medium and interpret reactions using suitable positive and negative controls. The supplement is used with a compatible medium base and does not serve as a complete stand-alone culture medium. Store the emulsion refrigerated at 2–8°C, protected from light, and inspect it for changes in appearance before use. The package contains one 100 mL preparation rather than powdered egg yolk or egg-yolk tellurite emulsion.
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